Please cite as:

CRISPRscan: Designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo
Miguel A. Moreno-Mateos*, Charles E. Vejnar*, Jean-Denis Beaudoin, Juan P. Fernandez, Emily K. Mis, Mustafa K. Khokha and Antonio J. Giraldez
*Equal contribution
Nature Methods 2015
doi:10.1038/nmeth.3543
PMID:26322839

Please cite, if you use…

SpG or SpRY

Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes
Jeremy Vicencio, Carlos Sanchez-Bolanos, Ismael Moreno-Sanchez, David Brena, Dmytro Kukhtar, Miguel Ruiz-Lopez, Mariona Cots-Ponjoan, Charles E. Vejnar, Alejandro Rubio, Natalia Rodrigo Melero, Carlo Carolis, Antonio J. Perez-Pulido, Antonio J. Giraldez, Benjamin P. Kleinstiver, Julian Ceron, Miguel Angel Moreno-Mateos
bioRXiv 2021
https://doi.org/10.1101/2021.06.06.447255

CFD score

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation
John G. Doench, Ella Hartenian, Daniel B. Graham, Zuzana Tothova, Mudra Hegde, Ian Smith, Meagan Sullender, Benjamin L. Ebert, Ramnik J. Xavier, and David E. Root
Nat Biotechnology 2014
doi:10.1038/nbt.3026
PMID:25184501