Tracks for zebrafish, medaka, xenopus, mouse and drosophila are available. Three classes of gRNA sites: (1) High, (2) low scores and (3) sites with off-targets.
All available gRNA sites predicted and scored with CRISPRscan within coding-sequence of protein-coding genes.
Submit your sequence to search gRNAs. Select your genome to predict off-targets.
All Cas9 gRNAs are scored with CRISPRscan model. Select gRNAs with highest score to increase your chance to get a mutant.
CRISPRscan includes predictions for zebrafish, Xenopus, human, mouse, Drosophila and more...
Refine your choice of gRNAs after exporting CRISPRscan predictions on your computer.
Whole genomes are searched for off-targets with up to 4 mismatches. Multiple scores and rules are available.
Protein domain annotations from Pfam, SMART, PROSITE, HMM-Panther, HAMAP etc are included in CRISPRscan.
Order the CRISPRscan provided oligo and generate your ready-to-inject sgRNA.