Now you can mutate (almost) anything
NEW in 2021
Improved graphical interface
Visualize gRNAs in genome context
More choices to search targets & off-targets
For in vitro transcribed or chemically synthesized gRNAs
Protein Domains in genome browser
Pfam, SMART, PROSITE etc
Please send us your feedback
charles [DOT] vejnar [AT] yale [DOT] edu
Search for gRNAs on genes
All available gRNA sites predicted and scored with CRISPRscan on all (coding or not) genes.
Predict gRNAs on your sequences
Submit your sequence to search gRNAs. Select your genome to predict off-targets.
Tracks for zebrafish, medaka, xenopus, mouse and drosophila are available. Three classes of gRNA sites: (1) High, (2) low scores and (3) sites with off-targets.
View browser tracks
Order the CRISPRscan provided oligo and generate your ready-to-inject sgRNA.
Whole genomes are searched for off-targets with up to 4 mismatches. Multiple scores and rules are available.
All gRNAs are presented in their genome context with genes and genomic sequence.
All Cas9 gRNAs are scored with CRISPRscan model. Select gRNAs with highest score to increase your chance to get a mutant.
CRISPRscan includes predictions for zebrafish, Xenopus, human, mouse, Drosophila and more...
Refine your choice of gRNAs after exporting CRISPRscan predictions on your computer.
Protein domain annotations from Pfam, SMART, PROSITE, HMM-Panther, HAMAP etc are included in CRISPRscan.