Protocol: Optimized CRISPR-Cas9 system for genome editing in zebrafish.


Mut-Seq pipeline installation

  1. Requirements
    • Python 3.2 (or more recent). A convenient Python installer like Anaconda can be used.
    • Download and install the GMAP read aligner.
  2. Download Mut-Seq package. Do not unzip it; this Python ZIP application contains Mut-Seq script and dependencies and can be used with Python directly (see example below). If needed, source code is available under the MPLv2 licence.

Mut-Seq example