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the coding sequence.
Locus Oligo for sgRNA generation
T7 promoterTarget
sequence
Tail annealing sequence
taatacgactcactataGGGTGTATTTGTGGAGCTTGgttttagagctagaa
taatacgactcactataGGCCTGGTCGATGGGATTCGgttttagagctagaa
taatacgactcactataGGAAGCACAGCACACGCCACgttttagagctagaa
...
Oligo extended with the tail:
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Oligo
Off-targets
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