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CRISPRscan score
>70 Highly efficient
>55 Efficient sgRNAs
Score
Locus Oligo for sgRNA generation
T7 promoterTarget
sequence
Tail annealing sequence
taatacgactcactataGGGTGTATTTGTGGAGCTTGgttttagagctagaa
taatacgactcactataGGCCTGGTCGATGGGATTCGgttttagagctagaa
taatacgactcactataGGAAGCACAGCACACGCCACgttttagagctagaa
...
Oligo extended with the tail:
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Oligo
Canonical and alternative sgRNAs
Canonical
Off-targets
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