Now you can mutate (almost) anything
Tracks for zebrafish, medaka, xenopus, mouse and drosophila are available. Three classes of sgRNA sites: (1) High, (2) low scores and (3) sites with off-targets.
Search for sgRNAs on genes
All available sgRNA sites predicted and scored with CRISPRscan within coding-sequence of protein-coding genes.
Predict sgRNAs on your sequences
Submit your sequence to search sgRNAs. Select your genome to predict off-targets.
Applying rules from Hsu et al. et Cong et al., whole genomes are searched for off-targets.
All sgRNAs are scored with CRISPRscan model. Select sgRNAs with highest score to increase your chance to get a mutant.
Refine your choice of sgRNAs after exporting CRISPRscan predictions on your computer.