CRISPRscan is a novel scoring algorithm from the Giraldez Lab (Yale University) that helps you select the best sgRNAs.
Now you can mutate (almost) anything

UCSC tracks

Tracks for zebrafish, medaka, xenopus, mouse and drosophila are available. Three classes of sgRNA sites: (1) High, (2) low scores and (3) sites with off-targets.

View UCSC tracks

Search for sgRNAs on genes

All available sgRNA sites predicted and scored with CRISPRscan within coding-sequence of protein-coding genes.

Target protein-coding genes

Predict sgRNAs on your sequences

Submit your sequence to search sgRNAs. Select your genome to predict off-targets.

Predict sgRNAs


Applying rules from Hsu et al. et Cong et al., whole genomes are searched for off-targets.

Select sgRNAs

All sgRNAs are scored with CRISPRscan model. Select sgRNAs with highest score to increase your chance to get a mutant.

Export sgRNAs

Refine your choice of sgRNAs after exporting CRISPRscan predictions on your computer.