Now you can mutate (almost) anything
Many new plants (Arabidopsis, soybean, rice, sorghum, corn)
Sea lamprey
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charles [DOT] vejnar [AT] gmail [DOT] com
Search for gRNAs on genes
All available gRNA sites predicted and scored with CRISPRscan on all (coding or not) genes.
Predict gRNAs on your sequences
Submit your sequence to search gRNAs. Select your genome to predict off-targets.
Genome browser
Tracks for zebrafish, medaka, xenopus, mouse and drosophila are available. Three classes of gRNA sites: (1) High, (2) low scores and (3) sites with off-targets.
gRNA generation
Order the CRISPRscan provided oligo and generate your ready-to-inject sgRNA.
Off-targets
Whole genomes are searched for off-targets with up to 4 mismatches. Multiple scores and rules are available.
Visualize gRNAs
All gRNAs are presented in their genome context with genes and genomic sequence.
Scores
All Cas9 gRNAs are scored with CRISPRscan model. Select gRNAs with highest score to increase your chance to get a mutant.
Multiple species
CRISPRscan includes predictions for zebrafish, Xenopus, human, mouse, Drosophila and more...
Export gRNAs
Refine your choice of gRNAs after exporting CRISPRscan predictions on your computer.
Protein domain
Protein domain annotations from Pfam, SMART, PROSITE, HMM-Panther, HAMAP etc are included in CRISPRscan.
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